Anti-mitotic Activities of Ethanolic Extract and Glutinol from Uvaria rufa Blume

Abstract: Background: This study describes the isolation of major chemical constituent from bark Uvaria rufa Blume, commonly known as “allagat or susong kalabaw”, an indigenous Philippine medicinal plant that belongs to family Annonaceae. It is a short climbing shrub grows in low and medium-altitude forests country. Materials Methods: The samples U. were air-dried for three months without exposure sunlight. ground blender, then, soaked CH2Cl2 ethanol days filtered. crude extract was chromatographed on gravity column dry packed with silica gel fractionated by chromatography using increasing proportion acetone at 10% increment. purified isolates subjected NMR structure elucidation. Their structures identified mainly 1H-NMR comparing current data those reported literature. Results: results showed dichloromethane afforded glutinol. Glutinol ethanolic exhibited anti-mitotic activity (18.33 % 18.93 %, respectively), comparable methotrexate (19.07 %), well-known anti-cancer drug even more potent which they surpassed 0.74 0.14 %. Conclusion: glutinol can be one main metabolites responsible plant. may also contained other substances might have great potential novel therapeutic agents cancer. Keywords: rufa, Allagat, Glutinol, Anti-mitotic, Triterpene.